High-pressure liquid chromatography

The abbreviation HPLC stands for high pressure liquid chromatography.

This method of analysis is used to separate low-volatile mixtures of substances. The dissolved substance is injected into the HPLC and is separated into its individual components under high pressure in a separating column. The peak signal generated by a detector is analysed by a computer. Modern HPLCs are capable of determining the quantities of one or more components.